Categories
Author Correction

Author Correction: Comprehensive profiling of circular RNAs with nanopore sequencing and CIRI-long

Correction to: Nature Biotechnology https://doi.org/10.1038/s41587-021-00842-6, published online 11 March 2021.In the version of this article initially published online, an additional PCR step was missing at the beginning of the last paragraph in the “Nanopore library preparation” section of the Methods: To obtain sufficient cDNA products for sequencing, PCR amplification was performed using 2 μL of…

Correction to: Nature Biotechnology https://doi.org/10.1038/s41587-021-00842-6, published online 11 March 2021.

In the version of this article initially published online, an additional PCR step was missing at the beginning of the last paragraph in the “Nanopore library preparation” section of the Methods: To obtain sufficient cDNA products for sequencing, PCR amplification was performed using 2 μL of cDNA with NEBNext LongAmp Taq DNA Polymerase and SMARTer primers under the following conditions: 95 °C for 30 s; 19 or 20 cycles of 95 °C for 15 s, 62 °C for 15 s, and 65 °C for 120 s; 65 °C for 6 min and hold at 4 °C. Also, the following has been added at the end of this section: Steps for library construction are detailed in the CIRI-long manual pages at https://ciri-cookbook.readthedocs.io/en/latest/CIRI-long_sequencing.html. The error has been corrected in the print, PDF and HTML versions of the article.

Author information

Author notes

  1. These authors contributed equally: Jinyang Zhang, Lingling Hou, Zhenqiang Zuo.

Affiliations

  1. Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, China

    Jinyang Zhang, Lingling Hou, Zhenqiang Zuo, Peifeng Ji & Fangqing Zhao

  2. University of Chinese Academy of Sciences, Beijing, China

    Jinyang Zhang & Fangqing Zhao

  3. Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China

    Xiaorong Zhang & Yuanchao Xue

  4. Center for Excellence in Animal Evolution and Genetics, Chinese Academy of Sciences, Kunming, China

    Fangqing Zhao

  5. Key Laboratory of Systems Biology, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Hangzhou, China

    Fangqing Zhao

Corresponding author

Correspondence to
Fangqing Zhao.

About this article

Verify currency and authenticity via CrossMark

Cite this article

Zhang, J., Hou, L., Zuo, Z. et al. Author Correction: Comprehensive profiling of circular RNAs with nanopore sequencing and CIRI-long.
Nat Biotechnol (2021). https://doi.org/10.1038/s41587-021-00934-3

Download citation

Read More

Leave a Reply

Your email address will not be published. Required fields are marked *